Single Cell Analysis – Differential Gene Expression

Single Cell Analysis – Differential Gene Expression

Single Cell Analysis – Differential Gene Expression

In Part I of our single cell analysis series, we introduced the Partek single-cell toolkit and focused on identification and characterization of cell populations. In this webinar, we will demonstrate how to handle a more complex study design, with multiple biological replicates, and how to detect genes that are differentially expressed between single-cell populations.

Agenda

  • Cell filtering using interactive QC charts
  • Identifying cell populations by t-SNE plot
  • Pooling cells of the same type across multiple samples
  • Detecting differentially expressed genes
  • Visualizing transcription profiles by heat map
  • Biological interpretation by GO or pathway enrichment

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Kathi GoscheSingle Cell Analysis – Differential Gene Expression