RNA-Seq Data Analysis Hands-on Training
Online Event
25 February 2021 | 3:00 p.m. – 5:00 p.m. SGT
Join us in learning the basics to analysing data from a RNA‑Seq experiment in Partek® Flow® bioinformatics software using simple point-and-click gestures. Attendees will work with a test dataset during this event. This course will be conducted online.
Please join the training from a computer running Google Chrome.
You will learn how to:
- Import RNA-Seq data from unaligned fastq files, aligned bam files and gene counts
- Generate and read pre alignment QA/QC report
- Trim adaptors and reads for good alignment outcome
- Generate and read post alignment QA/QC report
- Quantify reads to gene annotation database
- Generate and read quantification report
- Understand several RNA-Seq specific normalization methods
- Carry out differential gene analysis across multiple samples with false discovery rate correction
- Carry out gene ontology enrichment and pathway enrichment analysis
- Plot and edit visualizations for RNA-Seq: PCA, dot plot, heat map, volcano plot, pathway map